Fluorescent Protein Stability Assessment

This lecture discusses the use of RFP and GFP to assess protein stability. It covers topics such as flow cytometry, stability assessment, translation, and transcription correlation. The lecture also delves into mutagenesis experiments, calcium reporters, and RNA aptamers targeting viral proteins. The instructor explains the importance of nuclear localization sequences, protospacer motifs, and spacer sequences in CRISPR-Cas9 experiments. The session concludes with a discussion on reducing autofluorescence and optimizing experimental conditions for accurate results.