Enzyme Inhibitors: Drug Design and Tools

This lecture covers enzyme inhibitors as drugs, drug design, mechanisms of action, tools modulating enzyme activity, and protein function. It also discusses activity-based protein profiling, genome/transcriptome probing tools, and the use of 7-AAD in DNA intercalation. The instructor presents an in-class quiz on concepts from the previous week, focusing on NIH-3T3 cells and various treatments. The lecture explores biological questions that are challenging to solve with conventional tools, such as monitoring DNA replication and mapping DNA-protein interactions. It delves into the use of RNA aptamers, fluorescent proteins like GFP, and Spinach RNA mimics. The lecture showcases innovative applications of RNA aptamers in visualizing RNA, sensing metabolites, and tracking proteins in live cells.