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This lecture covers the concepts of biofabrication and bioprinting, including the automated generation of biologically functional products from living cells, bioactive molecules, and biomaterials. It explains the building blocks in biofabrication, various bioprinting technologies like inkjet printing and extrusion, and the challenges and advancements in creating complex structures. The lecture also delves into the use of sacrificial materials, viscosity enhancers, and multimaterial printing for creating intricate tissue constructs. Furthermore, it discusses in vivo and in situ printing, hybrid printing with dECM-derived bioinks, and the future prospects of bioprinting as a clinically-ready technology.