DNA Sequencing: Techniques and Applications

This lecture covers the evolution of DNA sequencing techniques, from the costly early methods to the current nanopore technology. It explains the process of shotgun sequencing, the challenges of understanding repetitive DNA sequences, and the importance of direct RNA sequencing. The lecture also delves into real-time PCR, the use of fluorescent molecules like Cybergreen and Taqman probes, and the principles behind measuring DNA concentration. It concludes with a detailed explanation of a compact, disposable system for real-time PCR, highlighting the importance of controlling temperature and measuring fluorescence in DNA analysis.