Lecture
This lecture introduces immunoassays, which measure substance concentration in biological liquids by binding capture and analyte molecules. It covers the reaction kinetics, configurations, and various techniques like immuno-agglutination, fluorescence polarization, ELISA, chemiluminescence, and fluorescence immunoassays. The lecture also discusses chromogenic enzymes, multiplex immunoassays using Luminex bead arrays, digital ELISA, and in-flow encoded particles for multiplex assays. Furthermore, it explores chemiluminescence using luminol and luciferin, as well as microflow immunoassays with silver signal amplification techniques.