Immunoassays: Basics and Techniques

This lecture introduces immunoassays, which measure substance concentration in biological liquids by binding capture and analyte molecules. It covers the reaction kinetics, configurations, and various techniques like immuno-agglutination, fluorescence polarization, ELISA, chemiluminescence, and fluorescence immunoassays. The lecture also discusses chromogenic enzymes, multiplex immunoassays using Luminex bead arrays, digital ELISA, and in-flow encoded particles for multiplex assays. Furthermore, it explores chemiluminescence using luminol and luciferin, as well as microflow immunoassays with silver signal amplification techniques.