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This lecture delves into the analysis of a 2D neuron model using pulse input. The instructor explains how pulse input can be used to understand spike generation in neurons, illustrating the process with the FitzHugh-Nagumo model. By injecting a short current pulse into the neuron, the voltage undergoes a jump to a new value, leading to different trajectories in the phase plane. The lecture demonstrates how different sizes of current pulses result in varying voltage trajectories, ultimately affecting spike generation in the neuron model.