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This lecture covers the principles and applications of Nuclear Magnetic Resonance (NMR), focusing on the elucidation of chemical structures and molecular dynamics. It explains the basics of NMR, including the spin of nuclei, magnetic moments, and the interpretation of NMR spectra. The lecture delves into topics such as chemical shift, peak integration, and peak multiplicity. It also discusses the use of NMR in studying complex molecules like proteins and the determination of protein structures. Additionally, it explores the use of NMR in medical imaging, particularly in Magnetic Resonance Imaging (MRI) for visualizing organs and tissues. The lecture concludes with an overview of NMR spectroscopy in three dimensions and its applications in various fields.