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Lecture
Design Optimization in ChemBio
Description
This lecture covers the design optimization of Snif-it's sensors, focusing on geometry, sequence of domains, spacing, and circular permutation, aiming to understand the molecular basis for changes in FRET ratio and the effective concentration of intra-molecular ligands. It also introduces semi-synthetic sensors for visualizing metabolite concentrations and LUCID's bioluminescent sensor proteins for drug monitoring, including the principles behind them and their applications in point-of-care therapeutic drug monitoring.
Official source
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