Lecture
This lecture introduces the concept of array tomography as a high-resolution imaging technique used to visualize individual neurons, axons, dendrites, and synapses in the brain. The instructor explains the principles behind array tomography, the process of embedding brain samples in resin, collecting ultra-thin sections, performing immunofluorescence staining, and reconstructing volumetric images. The lecture showcases examples of volumetric images highlighting different molecular elements within neural circuits, such as myelin basic protein, GABA, neurofilaments, and DAPI. Additionally, the integration of array tomography with scanning electron microscopy is discussed, demonstrating the ability to precisely register different imaging modalities and visualize synaptic structures in high detail.