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This lecture covers the structure and function of DNA, including DNA replication, transcription, and mRNA translation. It also discusses the regulation of gene expression and the process of manufacturing proteins in cells, highlighting the importance of RNA polymerase and transcription factors. The differences between prokaryotic and eukaryotic messengers, as well as the splicing of primary transcripts into mature mRNAs, are explained. The lecture also delves into the concept of alternative splicing, which allows for the generation of multiple mRNA variants from a single gene. Additionally, the role of RNA polymerase in transcription and the process of elongation are detailed, emphasizing the accuracy and efficiency of RNA synthesis.