Neuroscience Techniques: Two-Photon Microscopy

This lecture covers the principles and applications of two-photon microscopy in neuroscience, explaining how it overcomes light scattering in brain imaging, the point scanning technique used, and the benefits of intrinsic optical sectioning. It also delves into the importance of inactivating brain regions for behavior studies, trans-synaptic labeling with rabies virus, the role of mid-brain dopaminergic neurons in reward signaling, and somatotopic mapping in the neocortex. The instructor also addresses questions related to lab techniques and exam preparation.