Genetic Code Expansion: Tools and Techniques

This lecture covers the fundamentals of mRNA translation, including bacterial and eukaryotic mRNA structures, translation stages, and tRNA secondary structures. It also delves into translational blockers, mRNA display technology, and genetic code expansion methods like amber suppression. The instructor discusses the SILAC-BONCAT technique, global unnatural amino acid incorporation, and the BONCAT strategy for labeling newly synthesized proteins. Practical applications of genetic code expansion in the mouse brain and the survey of amino acids incorporated via unnatural amino acid mutagenesis are also explored.