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This lecture covers the principles of electrochemical biosensors, including potentiometric, amperometric, and conductometric biosensors. It explains the Butler-Volmer equation, the Clark electrode for blood oxygen sensing, depletion layers, enzymatic amperometric biosensors, and glucose sensors. The use of mediators, cyclic voltammetry, ultra microelectrodes, and redox cycling in amperometry are also discussed, along with applications like continuous glucose monitoring and immunoassays. The lecture concludes with electrochemiluminescence and its application in biosensors, exemplified by the Roche Cobas system.