Correlative Imaging Techniques: CryoSEM and NanoSIMS Applications

This lecture discusses advanced imaging techniques, specifically correlative cryo-scanning electron microscopy (cryoSEM) and nanoscale secondary ion mass spectrometry (NanoSIMS), for studying biological tissues. The instructor introduces the principles of NanoSIMS, highlighting its ability to create detailed chemical maps of elemental and isotopic distributions within samples. The lecture emphasizes the importance of high-resolution imaging in biological research, particularly in understanding cellular responses to salinity stress in plants. The workflow for cryoNanoSIMS is outlined, detailing the steps from sample preparation to imaging. The instructor presents case studies demonstrating how these techniques can visualize sodium and potassium distributions in plant cells under varying salt stress conditions. The potential of cryoNanoSIMS to revolutionize biological imaging is underscored, showcasing its ability to provide insights into subcellular processes and the interactions of different ions within cells. The lecture concludes with a discussion on future applications and the ongoing developments in imaging technologies.