Hsp70 Mechanism: Unfolding & Entropic Pulling

This lecture delves into the intricate mechanism of Hsp70, exploring its role in protein unfolding and the concept of entropic pulling. Through a series of slides, the instructor discusses the evolution of knowledge in the field over the past two decades, the thermodynamic considerations of protein folding, the action of chaperones, and the experimental evidence supporting the chaperone-driven stabilization of native proteins. The lecture also covers the use of ATP hydrolysis to enhance Hsp70's affinity for substrates, the non-equilibrium stabilization of native proteins, and the potential synergy between J-Domain Proteins and Hsp70. The presentation concludes with future research directions and acknowledgments to collaborators and funding sources.