Fluorescence in Microscopy: Introduction & Labeling Techniques

This lecture introduces fluorescence in microscopy, covering different types of fluorophores such as organic dyes, fluorescent proteins, quantum dots, and lanthanides. It explains methods for introducing fluorescence labels, including linking dye molecules to specific antigens, crosslinking with covalent bonding, and immunofluorescent staining. The lecture also discusses the specificity of fluorescence markers for targeting cell structures, multi-color imaging, concerns in multi-color fluorescence like photobleaching, and the influence of environmental factors on fluorescence properties.