Evolution of T7 RNAP Specificity

In course

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Description

This lecture discusses the continuous evolution of T7 RNA polymerase (T7 RNAP) variants to recognize different promoters, focusing on the PACE system. The lecture covers the process of inducing mutations in T7 RNAP genes, the activity improvements observed, and the specific mutations identified in the evolved variants.

Instructor

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Official source

https://mediaspace.epfl.ch/media/0_zsaypovo

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Ontological neighbourhood

Biology

Genetics: Molecular genetics

Molecular biology: Topics in molecular biology

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