This lecture by the instructor covers the concept of cell type specific connectivity and shotgun connectomics, focusing on the challenges and techniques involved in mapping neural connections at different scales. The presentation delves into the methods used to study connectivity patterns in the brain, from dense mm³ connectomics to sparse shotgun connectomics, highlighting the differences in sampling density and measurement scale. The lecture also discusses the application of array tomography, technical issues in large-scale imaging, and the importance of volume assembly in understanding neural circuits.