Publications by Jean-Baptiste Perez

Cell Membranes Suspended Across Nanoaperture Arrays

Jürgen Brugger, Horst Vogel, Christian Santschi, Christophe Danelon, Jean-Baptiste Perez

The authors present a method for spreading large (> 100 mm2) cell membrane fragments across nanoapertures in planar supports. Electron-beam and focused-ion-beam lithog. were used to fabricate arrays of 50-600 nm diam. holes in free-standing silicon nitride ...

2006

Supported cell-membrane sheets for functional fluorescence imaging of membrane proteins

Horst Vogel, Jean-Manuel Segura, Jean-Baptiste Perez

Cell-membrane sheets suitable for in-vitro functional fluorescence studies have been prepd. by direct detachment from cell membranes using poly-L-lysine-coated glass slides. The resulting transferred planar membranes conserve the compn. as well as most pro ...

2006

Micro- and nanostructured devices for investigating biomolecular interactions

Jürgen Brugger, Horst Vogel, Christian Santschi, Christophe Danelon, Jean-Baptiste Perez, Christoph Schreiter

Cell membrane receptors and ion channels are essential in many different cellular processes. To analyse the activity of membrane proteins in vesicles and biological cells, we fabricated micro- nanostructured chips, enabling the application of electrophysio ...

2006

Monitoring the Diffusion of Single Heterotrimeric G Proteins in Supported Cell-membrane Sheets Reveals their Partitioning into Microdomains

Horst Vogel, Jean-Manuel Segura, Joachim Piguet, Jean-Baptiste Perez

Supported cell-membrane sheets are promising in vitro systems to investigate the properties of membranes with native protein/lipid composition, in particular their sub-compartmentalization and the differential localization of proteins associated to them. W ...

Elsevier2006

Investigating molecular interactions of G protein coupled receptors by fluorescence techniques

Jean-Baptiste Perez

G Protein coupled receptors (GPCRs) constitute an abundant family of membrane proteins which play a central role in cellular signaling and are important targets for modern medicine. Despite intensive research, central questions of the molecular mechanism o ...

EPFL2005