Thomas Rizzo

Biography

EDUCATION

Ph.D., Chemistry, University of Wisconsin, Madison, 1983

B.S., Chemistry, cum laude, Rensselaer Polytechnic Institute, 1978

ACADEMIC AND ADMINISTRATIVE POSITIONS

Dean, Faculty of Basic Sciences, EPFL, 2004-present

Head, Department of Chemistry, EPFL, 1997-2004

Professor of Chemistry, EPFL, 1994-present

Professor of Chemistry, University of Rochester, 1993-1994

Assistant Professor of Chemistry, University of Rochester, 1986-1992

Research Associate, The James Franck Institute, University of Chicago, 1984-1986

Official source

https://people.epfl.ch/thomas.rizzo

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Courses taught by this person (2)

Cet enseignement vise l'acquisition des notions essentielles relatives à la structure de la matière, aux équilibres et à la réactivité chimiques. Le cours et les exercices fournissent la méthodologie permettant de résoudre par le raisonnement et le calcul des problèmes inédits de chimie générale.

This course aims to teach essential notions of the structure of matter, chemical equilibria and reactivity. Classes and exercises provide the means to analyze and solve, by reasoning and calculation, novel problems of geeneral chemistry.

Courses taught by this person

Related research domains (95)

Related research domains

Related publications (164)

Related publications

People doing similar research (94)

People doing similar research

Related publications (164)

Identification of human milk oligosaccharide positional isomers by combining IMS-CID-IMS and cryogenic IR spectroscopy

Ahmed Ben Faleh, Thomas Rizzo, Stephan Warnke, Vasyl Yatsyna

High-resolution ion mobility spectrometry (IMS) coupled with cryogenic infrared spectroscopy has proven to be a powerful technique for the identification of oligosaccharides. However, the need for an extensive database, combined with the scarcity of pure standards, remains a significant barrier to the broad application of this approach. To solve this issue, we demonstrate a method in which ion fragments produced by collision-induced dissociation (CID) are separated using IMS and identified using the vibrational fingerprints of only a few standards. Identification of the fragments allows us to determine the structure of the precursor molecule, the vibrational fingerprint of which is then added to our database. We then show how we can use this approach to identify the structure of mobility separated isomers found in pooled human milk.

ROYAL SOC CHEMISTRY2023

Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis

Priyanka Bansal, Ahmed Ben Faleh, Thomas Rizzo, Stephan Warnke

The structural complexity of glycans makes their characterization challenging, not only because of the presence of various isomeric forms of the precursor molecule but also because the fragments can themselves be isomeric. We have recently developed an IMS-CID-IMS approach using structures for lossless ion manipulations (SLIM) combined with cryogenic infrared (IR) spectroscopy for glycan analysis. It allows mobility separation and collision-induced dissociation of a precursor glycan followed by mobility separation and IR spectroscopy of the fragments. While this approach holds great promise for glycan analysis, we often encounter fragments for which we have no standards to identify their spectroscopic fingerprint. In this work, we perform proof-of principle experiments employing a multistage SLIM-based IMS-CID technique to generate second-generation fragments, followed by their mobility separation and spectroscopic interrogation. This approach provides detailed structural information about the first generation fragments, including their anomeric form, which in turn can be used to identify the precursor glycan.

AMER CHEMICAL SOC2023

New Approach for the Identification of Isobaric and Isomeric Metabolites

Ahmed Ben Faleh, Thomas Rizzo, Stephan Warnke

The structural elucidation of metabolite molecules is important in many branches of the life sciences. However, the isomeric and isobaric complexity of metabolites makes their identification extremely challenging, and analytical standards are often required to confirm the presence of a particular compound in a sample. We present here an approach to overcome these challenges using high-resolution ion mobility spectrometry in combination with cryogenic vibrational spectroscopy for the rapid separation and identification of metabolite isomers and isobars. Ion mobility can separate isomeric metabolites in tens of milliseconds, and cryogenic IR spectroscopy provides highly structured IR fingerprints for unambiguous molecular identification. Moreover, our approach allows one to identify metabolite isomers automatically by comparing their IR fingerprints with those previously recorded in a database, obviating the need for a recurrent introduction of analytical standards. We demonstrate the principle of this approach by constructing a database composed of IR fingerprints of eight isomeric/isobaric metabolites and use it for the identification of these isomers present in mixtures. Moreover, we show how our fast IR fingerprinting technology allows to probe the IR fingerprints of molecules within just a few seconds as they elute from an LC column. This approach has the potential to greatly improve metabolomics workflows in terms of accuracy, speed, and cost.

AMER CHEMICAL SOC2023