Person

Henning Paul-Julius Stahlberg

Related publications (206)

Projection Structure of the Secondary Citrate/Sodium Symporter CitS at 6 angstrom Resolution by Electron Crystallography

CitS from Klebsiella pneumoniae acts as a secondary symporter of citrate and sodium ions across the inner membrane of the host. The protein is the best characterized member of the 2-hydroxycarboxylate transporter family, while no experimental structural in ...

Elsevier BV2012

Limiting factors in single particle cryo electron tomography

Henning Paul-Julius Stahlberg

Modern methods of cryo electron microscopy and tomography allow visualization of protein nanomachines in their native state at the nanometer scale. Image processing methods including sub-volume averaging applied to repeating macromolecular elements within ...

Elsevier BV2012

Assessing the benefits of focal pair cryo-electron tomography

Henning Paul-Julius Stahlberg

Cryo electron tomography provides nanometer-scale information on biological matter preserved in a close-to native state. The resolution of tomograms and structures resolved by sub-tomogram averaging is typically limited by the contrast transfer function of ...

Elsevier BV2012

Merging of image data in electron crystallography

Henning Paul-Julius Stahlberg

Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the re ...

Humana Press2012

Structure and Function of Purified Monoclonal Antibody Dimers Induced by Different Stress Conditions

Henning Paul-Julius Stahlberg, Alexandre Briguet

To investigate structure and function of different monoclonal antibody (MAb) dimers.MAb dimers were induced by process-related, low pH and UV light stress. Dimers were isolated and purified by chromatography and extensively characterized by biochemical, st ...

Springer Nature2012

The application of graphene as a sample support in transmission electron microscopy

Henning Paul-Julius Stahlberg, Radosav Svetozara Pantelic

Transmission electron microscopy has witnessed rampant development and surging point resolution over the past few years. The improved imaging performance of modern electron microscopes shifts the bottleneck for image contrast and resolution to sample prepa ...

Elsevier BV2012

Ionic liquids as matrices in microfluidic sample deposition for high-mass matrix-assisted laser desorption/ionization mass spectrometry

Henning Paul-Julius Stahlberg

Sample preparation for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) via a microfluidic deposition device using ionic liquid matrices addresses several problems of standard protocols with crystalline matrices, such as the heterog ...

IM Publications2012

Characterization of the motion of membrane proteins using high-speed atomic force microscopy

Henning Paul-Julius Stahlberg

For cells to function properly(1), membrane proteins must be able to diffuse within biological membranes. The functions of these membrane proteins depend on their position and also on protein-protein and protein-lipid interactions(2). However, so far, it h ...

Springer Nature2012

Connecting mu-fluidics to electron microscopy

Henning Paul-Julius Stahlberg, Andreas Engel

A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic wr ...

Elsevier BV2012

Automation of image processing in electron crystallography

Henning Paul-Julius Stahlberg

Electron crystallography of membrane proteins records images and diffraction patterns of frozen-hydrated two-dimensional (2D) crystals. To reconstruct the high-resolution three-dimensional (3D) structure of a membrane protein, a multitude of images of 2D c ...

Humana Press2012

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