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The functional anal. of single ion channel proteins presents a serious bottleneck in the process of finding new pharmacol. active compds. Currently available single channel recording methods are not suited for automation and miniaturization. However, new t ...
A review. Cell-based assays are the predominant means of assessing activation of ionotropic receptors and G protein-coupled receptors (GPCRs), but are limited by their inherent complexity. Here we present an approach to immobilize receptors stably and with ...
The binding of the fluorescein-labeled antagonist GR-flu ([1,2,3,9-tetrahydro-3-[(5-methyl-1H-imidazol-4-yl)methyl]-9-(3-amino- (N-fluoresceinthiocarbamoyl)propyl)-4H-carbazol-4-one]) to a purified, detergent-solubilized ligand-gated ion channel, the type- ...
The std. deviation (SD) in fluorescence correlation spectroscopy (FCS) has been mostly neglected in applications. However, the knowledge of the correct SD is necessary for an accurate data evaluation, esp. when fitting theor. models to exptl. data. In this ...
Fluorescence Correlation Spectroscopy (FCS) is a suitable technique to investigate biomol. interactions. The advantage of FCS lies in its wide dynamic range in the time and concn. domain, its sensitivity, and thus in the small amt. of material needed. The ...
Information on the temporal order, the kinetics, and the energetics of each of the reaction intermediates is required in order to fully elucidate the mechanism of catalytic interaction of G proteins with their receptors. Intrinsic monitors, such as light s ...
The nicotinic acetylcholine receptor from Torpedo was immobilised in tethered membranes. Surface plasmon resonance was used to quantify the binding of ligands and antibodies to the receptor. The orientation and structural integrity of the surface-reconstit ...
The expression of recombinant proteins with the Semliki Forest Virus (SFV) system has been scaled up to bioreactor scale. As a model protein for this study the human 5-HT(3) receptor was chosen. The gene for the receptor was subcloned into the SFV expressi ...
The ability of organisms, or individual cells to sense and to react to different external and internal stimuli (light, hormones, odorants, taste) is crucial for their survival. I will report on our recent progress to investigate mol. signal recognition and ...
A new strategy for the reversible formation of supported lipid bilayers is introduced. It uses an extension of immobilized metal ion affinity chromatog. technol., which depends on the reversible formation of complexes between metal ion binding chelator gro ...
