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Erwin Ilegems

Related publications (7)

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Conformational changes of channel activation: Five enhanced green fluorescent protein (EGFP) mols. (green cylinders) were integrated into the intracellular part of the homopentameric ionotropic 5-HT3 receptor. This allowed the detection of extracellular bi ...

2005

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Understanding cellular signaling mediated by cell surface receptors is key to modern biomedical research and drug development. The discovery of a growing no. of potential mol. targets and therapeutic compds. requires downscaling and accelerated functional ...

2005

In vivo protein labeling for structural and functional investigation of the 5-HT3A neurotransmitter receptor

Erwin Ilegems

In this thesis, two different fluorescent labeling techniques for in vivo investigations on the 5-HT3 receptor (5-HT3R) functions are presented. This plasma membrane protein contains five subunits surrounding an ion channel that opens after binding of a 5- ...

EPFL2004

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Sequential stages in the life cycle of the ionotropic 5-HT3 receptor (5-HT3R) were resolved temporally and spatially in live cells by multicolor fluorescence confocal microscopy. The insertion of the enhanced cyan fluorescent protein into the large intrace ...

2004

Downregulation of eRF1 by RNA interference increases mis-acylated tRNA suppression efficiency in human cells

Horst Vogel, Horst Pick, Erwin Ilegems

The site-specific incorporation of non-natural amino acids into proteins by nonsense suppression has been widely used to investigate protein structure and function. Usually this technique exhibits low incorporation efficiencies of non-natural amino acids i ...

2004

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High signal-to-noise Fourier transform IR (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT3R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein ...

2003

Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery

Horst Vogel, Horst Pick, Erwin Ilegems

A reporter assay was developed to detect and quantify nonsense codon suppression by chem. aminoacylated tRNAs in mammalian cells. It is based on the cellular expression of the enhanced green fluorescent protein (EGFP) as a reporter for the site-specific am ...

Oxford University Press2002