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The ultrafast spectral evolution of the fluorescence of the all-trans protonated Schiff base of retinal (PSBR) in alcs. with different polarity was studied using the fluorescence up-conversion technique in a "polychromatic mode". Results reveal that vibr ...
Time-integrated fluorescence expts. on native bacteriorhodopsin and on its non-isomerizing form bR5.12 are reported. The exptl. set-up was designed such as to observe emission exclusively from the excited state intermediate I-460. We obtain the first syste ...
Absorption, fluorescence and excitation spectra of three-dimensional bacteriorhodopsin crystals harvested from a lipidic cubic phase are presented. The combination of the spectroscopic expts. performed at room temp., controlled pH and full external hydrati ...