Person
Fernando Cortes Salazar
This person is no longer with EPFL
Related publications (43)
The antioxidant capacity of erythrocyte concentrates is increased during the first week of storage and correlated with the uric acid level
Hubert Girault, Niels Erik Lion, Andreas Stephan Lesch, Fernando Cortes Salazar, Michel Prudent, Sunny Isaïe Maye, Manon Sandra Bardyn
Background and Objectives: Red blood cells (RBCs) suffer from lesions during cold storage, depending in part on their ability to counterbalance oxidative stress by activating their antioxidant defence. The aim of this study was to monitor the antioxidant p ...
Wiley-Blackwell2017Inkjet-printed microtiter plates for portable electrochemical immunoassays
Hubert Girault, Frédéric Gumy, Andreas Stephan Lesch, Fernando Cortes Salazar, Milica Jovic, Yingdi Zhu, Alexandra Bondarenko
Herein, we present the large-scale fabrication of multiplexed three-electrode sensors used in a point-of-care device platform that couples a magnetic bead-based immunoassay strategy with amperometric detection for rapid and highly sensitive analysis. The m ...
Elsevier Science Sa2017Characterization of Surface State of Inert Particles: Case of Si and SiC
Hubert Girault, Fernando Cortes Salazar, Véronique Amstutz, Desire Martial Koutouan Abro
Silicon and Silicon carbide particles have been investigated by the mean of infrared (IR) spectroscopy and X-ray photoelectron spectroscopy (XPS) to establish their surface states. The results of this research are based on the estimation of the area under ...
2016Monitoring Tyrosinase Expression in Non-metastatic and Metastatic Melanoma Tissues by Scanning Electrochemical Microscopy
Hubert Girault, Horst Pick, Andreas Stephan Lesch, Fernando Cortes Salazar, Tzu-En Lin, Alexandra Bondarenko
Although tremendous progress has been made in the diagnosis of melanoma, the identification of different stages of malignancy in a reliable way remains challenging. Current strategies rely on optical monitoring of the concentration and spatial distribution ...
Wiley-Blackwell2016Untersuchung der Tyrosinase-Expression in nicht-metastatischen und metastatischen Melanomgeweben durch elektrochemische Rastersondenmikroskopie
Hubert Girault, Horst Pick, Andreas Stephan Lesch, Fernando Cortes Salazar, Tzu-En Lin, Alexandra Bondarenko
Trotz des enormen Fortschritts, der bei der Diagnose von Melanomen erzielt wurde, bleibt die zuverlässige Erkennung der verschiedenen Malignomstadien eine Herausforderung. Derzeitige Strategien setzen auf die optische Erfassung der Konzentration und räumlichen Verteilung spezifischer Biomarker. Neueste optische Methoden können allerdings durch Interferenzen mit Hintergrundfarben und durch Autofluoreszenz beeinflusst werden. Wir bewältigen diese Defizite hier unter Einsatz der elektrochemischen Rastersondenmikroskopie (SECM), um den prognostischen Indikator Tyrosinase (TyR) in nicht-metastatischen und metastatischen Melanomgeweben mithilfe einer weichen Mikroelektrodensonde abzubilden. Das elektrochemische Auslesen der TyR-Verteilung wurde durch das Adaptieren eines immunchemischen Verfahrens ermöglicht. Wir zeigen, dass die SECM die erwähnten Beschränkungen optischer Methoden übertreffen kann und bisher nicht gekannte Möglichkeiten für eine verbesserte Diagnose und das Verständnis der räumlichen Verteilung von TyR in verschiedenen Melanomstadien eröffnet.
Wiley-Blackwell2016Aluminium Foil as Single-Use Substrate for MALDI-MS Fingerprinting of Different Melanoma Cell Lines
Hubert Girault, Horst Pick, Liang Qiao, Fernando Cortes Salazar, Yingdi Zhu, Alexandra Bondarenko
Herein, we present the intact cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the fingerprinting of human melanoma cancer cell lines grown on aluminium foil. To perform the MALDI-MS assay, melanoma cells were cultured on a ...
Royal Soc Chemistry2016Fixation and Permeabilization Approaches for Scanning Electrochemical Microscopy of Living Cells
Hubert Girault, Horst Pick, Andreas Stephan Lesch, Fernando Cortes Salazar, Tzu-En Lin, Alexandra Bondarenko, Petar Stupar
Scanning electrochemical microscopy (SECM) has been widely used for the electrochemical imaging of dynamic topographical and metabolic changes in alive adherent mammalian cells. However, extracting intracellular information by SECM is challenging, since it ...
American Chemical Society2016Electrochemical push-pull probe: from scanning electrochemical microscopy (SECM) to multimodal altering of cell microenvironment
Hubert Girault, Andreas Stephan Lesch, Fernando Cortes Salazar, Dmitry Momotenko, Alexandra Bondarenko
To understand biological processes at the cellular level, a general approach is to alter the cells’ environment and to study their chemical responses. Herein, we present the implementation of an electrochemical push-pull probe, which combines a microfluidi ...
Amer Chemical Soc2015Inkjet Printed Nanohydrogel Coated Carbon Nanotubes Electrodes For Matrix Independent Sensing
Hubert Girault, Andreas Stephan Lesch, Fernando Cortes Salazar, Véronique Amstutz
Polyacrylamide (PA) based hydrogels are used in several applications including polyacrylamide gel electrophoresis and sensing devices. Homogeneous and compact PA films can be prepared based on chemical or photopolymerization processes. However, the accurat ...
2015Multiple scanning electrochemical microscopy mapping of tyrosinase in micro-contact printed fruit samples on polyvinylidene fluoride membrane
Hubert Girault, Andreas Stephan Lesch, Liang Qiao, Fernando Cortes Salazar, Tzu-En Lin, Alexandra Bondarenko
Herein, we introduce three orthogonal and compatible methods for detecting tyrosinase, a key factor in fruit browning and skin disorders, with high spatial resolution by means of scanning electrochemical microscopy (SECM). All methods are performed subsequ ...
Elsevier2015