Person
Stewart Cole
Related publications (401)
Genomic organization of the mycobacterial sigma gene cluster
We have previously described sigma A and sigma B and their structural genes, mysA and mysB, respectively, in Mycobacterium smegmatis. We have now sequenced the corresponding regions in the M. tuberculosis and M. leprae chromosomes, and have found the two h ...
1995Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50.
The physical map of the 3.6-megabase chromosome of Clostridium perfringens CPN50 was extended by positioning sites for the endonucleases SfiI and I-CeuI, and in parallel, the gene map was expanded by using a genome scanning strategy. This involved the clon ...
1995The enterotoxin gene (cpe) of Clostridium perfringens can be chromosomal or plasmid-borne
The location of the cpe gene, encoding the enterotoxin responsible for food poisoning in humans, has been studied in a series of enterotoxigenic Clostridium perfringens strains by means of pulsed field gel electrophoresis of genomic DNA. The cpe gene was f ...
1995Mycobacterium tuberculosis: drug-resistance mechanisms
Tuberculosis has resurged during the past decade in many industrialized countries, and strains of Mycobacterium tuberculosis that are resistant to one or more of the main antituberculous drugs are emerging. The molecular basis of mycobacterial drug resista ...
1994Implications of multidrug resistance for the future of short-course chemotherapy of tuberculosis: a molecular study
Tuberculosis-control programmes are compromised by the increased frequency of multidrug-resistant strains of Mycobacterium tuberculosis. We used the polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP) analysis techniques to e ...
1994Purification, characterization, gene sequence, and significance of a bacterioferritin from Mycobacterium leprae.
The study of tissue-derived Mycobacterium leprae provides insights to the immunopathology of leprosy and helps identify broad molecular features necessary for mycobacterial parasitism. A major membrane protein (MMP-II) of in vivo-derived M. leprae previous ...
1994MycDB: an integrated mycobacterial database
As part of ongoing efforts to investigate the molecular biology of the human pathogens in the genus Mycobacterium, a customized database was developed specifically for these organisms and implemented in ACEDB database manager software. The data loaded incl ...
1994Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes and detection of quinolone resistance mutations.
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis has resulted in increased interest in the fluoroquinolones (FQs) as antituberculosis agents. To investigate the frequency and mechanisms of FQ resistance in M. tuberculosis, we clon ...
1994Streptomycin resistance in mycobacteria.
Streptomycin, the first antibiotic used in tuberculosis control programs, perturbs protein synthesis at the ribosome level. It is shown here that streptomycin resistance in some clinical isolates of Mycobacterium tuberculosis is associated either with miss ...
1994Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens.
A recombinant lambda phage was identified in a Clostridium perfringens genomic library by means of its ability to hydrolyse the fluorescent substrate 4-methyl-umbelliferyl-beta-D-glucosaminide, isolated and shown to encode an endo-beta-N-acetylglucosaminid ...
1994