Person

Stewart Cole

Related publications (401)

The sulA gene of Escherichia coli, which encodes an inducible inhibitor of cell division, plays a role in the SOS response. Its expression, like that of other SOS genes, is repressed by the LexA protein. This paper reports the identification and characteri ...

1983

Location and nucleotide sequence of frdB, the gene coding for the iron-sulphur protein subunit of the fumarate reductase of Escherichia coli

Stewart Cole

The frdB gene, encoding the iron-sulphur protein subunit of fumarate reductase, has been located and its complete nucleotide sequence determined. The identity of the gene was confirmed by protein chemical studies and determination of the NH2-terminal seque ...

1982

The nucleotide sequence coding for major outer membrane protein OmpA of Shigella dysenteriae

Stewart Cole

The nucleotide sequence of the ompA gene from Shigella dysenteriae has been determined and the amino acid sequence of the pro-OmpA protein predicted. Sequence comparison between the ompA genes of S.dysenteriae and Escherichia coli showed that features such ...

1982

Nucleotide sequence coding for the flavoprotein subunit of the fumarate reductase of Escherichia coli

Stewart Cole

The nucleotide sequence of the frdA gene, which encodes the flavoprotein subunit of the fumarate reductase, of Escherichia coli, has been determined. A polypeptide of Mr = 66,052, containing 602 amino acid residues, is predicted. In composition the FrdA pr ...

1982

Export of a protein into the outer membrane of Escherichia coli K12: Stable incorporation of the OmpA protein requires less than 193 amino-terminal amino-acid residues

Stewart Cole

The cloned ompA gene encoding the major outer membrane protein OmpA of Escherichia coli has been shortened in vitro by exonuclease digestion from the end corresponding to the CO2H terminus of the protein. Nine derivatives were identified which still posses ...

1982

Cloning and expression in Escherichia coli K-12 of the genes for major outer membrane protein OmpA from Shigella dysenteriae, Enterobacter aerogenes, and Serratia marcescens.

Stewart Cole

The outer membranes of many gram-negative bacteria contain a major heat-modifiable protein which shows serological cross-reactivity with the OmpA protein of Escherichia coli K-12. Using the cloned gene for the E. coli K12 protein as a DNA-DNA hybridization ...

1982

Characterisation of the promoters for the ompA gene which encodes a major outer membrane protein of Escherichia coli

Stewart Cole

The regulatory region of the ompA gene from Escherichia coli has been characterized by biochemical and genetic approaches. Two overlapping promoters, P1 and P2, organized in that order with respect to the ompA coding sequence, were identified and it was fo ...

1982

The organization and expression of the pyruvate dehydrogenase complex genes, aceE (E1), aceF (E2) and lpd (E3), were investigated using a series of λ transducing phages carrying different segments of the nadC-aroP-aceE-aceF-lpd region of the chromosome of ...

1981

Thermoinducible lysis-defective derivatives of λfrdA phages (λG1F and λG40F) carrying the fumarate marate reductase gene of Escherichia coli, inserted in each of two possible orientations, were used to amplify fumarate reductase synthesis and study the aer ...

1980

Two types of fumarate reductase transducing phages, lambda frdA, carrying the wild-type frdA gene but differing in the orientation of a R.HindIII fragment of bacterial DNA were isolated from populations of recombinant transducing phages by their ability to ...

1980