Publications by Chia-Lung Hsieh

Tissue and cellular imaging

Demetri Psaltis, Ye Pu, Ye Pu, Chia-Lung Hsieh, Xin Yang

Systems and methods for imaging ear tissue include: directing illumination radiation to pass through an intact biological structure and be incident on ear tissue that does not include an exogenous fluorophore, at a plurality of locations, the illumination ...

2013

Two-photon microscopy of the mouse cochlea in situ for cellular diagnosis

Demetri Psaltis, Ye Pu, Ye Pu, Chia-Lung Hsieh, Xin Yang

Sensorineural hearing loss is the most common type of hearing loss worldwide, yet the underlying cause is typically unknown because the inner ear cannot be biopsied today without destroying hearing, and intracochlear cells have not been imaged with resolut ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Three-dimensional scanning microscopy through thin turbid media

Demetri Psaltis, Ye Pu, Ye Pu, Chia-Lung Hsieh, Xin Yang

We demonstrate three-dimensional imaging through a thin turbid medium using digital phase conjugation of the second harmonic signal emitted from a beacon nanoparticle. The digitally phase-conjugated focus scans the volume in the vicinity of its initial pos ...

2012

Imaging with second-harmonic radiation probes in living tissue

Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh, Thomas Jean Victor Marie Lanvin

We demonstrate that second-harmonic radiation imaging probes are efficient biomarkers for imaging in living tissue. We show that 100 nm and 300 nm BaTiO3 nanoparticles used as contrast markers could be detected through 50 mu m and 120 mu m of mouse tail ti ...

2011

Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle

Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh, Grégoire Pierre Jean Laporte

We demonstrate imaging through a turbid layer by using digital phase conjugation of the second harmonic field radiated from a beacon nanoparticle. We show that the phase-conjugated focus can be displaced from its initial position by illuminating the same r ...

2010

Second harmonic generation from nanocrystals under linearly and circularly polarized excitations

Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We study second harmonic generation (SHG) from noncentrosymmetric nanocrystals under linearly polarized (LP) and circularly polarized (CP) excitations. Theoretical models are developed for SHG from nanocrystals under both plane-wave and focused excitations ...

2010

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal s ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Nonlinear optical properties of core-shell nanocavities for enhanced second-harmonic generation

Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh

A nonlinear optical plasmonic core-shell nanocavity is demonstrated as an efficient, subwavelength coherent light source through second-harmonic generation. The nonlinear optical plasmonic nanocavity incorporates a noncentrosymmetric medium, which utilizes ...

2010

Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging: erratum

Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh

An error was made in calculating the polarization dependent second harmonic response of barium titanate nanoparticles. We have corrected the error and repeated the comparison with the experimental results. (C) 2010 Optical Society of America ...

2010

Bioconjugation of barium titanate nanocrystals with immunoglobulin G antibody for second harmonic radiation imaging probes

Demetri Psaltis, Ye Pu, Ye Pu, Rachel Grange, Chia-Lung Hsieh

The second harmonic generation (SHG) active nanocrystals have been demonstrated as attractive imaging probes in nonlinear microscopy due to their coherent, non-bleaching and non-blinking signals with a broad flexibility in the choice of excitation waveleng ...

Elsevier2010