Lara Buscemi Estefanell

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The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

Jean-Jacques Meister, Hicham Majd, Lara Buscemi Estefanell, Boris Hinz, Pierre Jean Arnould Wipff

Strain devices with expandable polydimethylsiloxane (PDMS) culture membranes are frequently used to stretch cells in vitro, mimicking mechanically dynamic tissue environments. To immobilize cell-adhesive molecules to the otherwise non-adhesive PDMS substra ...

Elsevier2009

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Hicham Majd, Lara Buscemi Estefanell, Boris Hinz, Pierre Jean Arnould Wipff

Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

2008