Person

Virginie Hamel

This person is no longer with EPFL

Related publications (13)

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly

Georg Fantner, Georgios Hatzopoulos, Tatiana Favez, Oliver Hantschel, Virginie Hamel, Niccolo Banterle, Santiago Harald Andany

Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that sca ...

NATURE RESEARCH2021

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Khalid Ashraf Mohie Ibrahim, Davide Gambarotto, Paul Guichard, Virginie Hamel, Denis Fortun, Niccolo Banterle, Dora Mahecic

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

NATURE PUBLISHING GROUP2020

A helical inner scaffold provides a structural basis for centriole cohesion

Henning Paul-Julius Stahlberg, Davide Gambarotto, Paul Guichard, Virginie Hamel

The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary ...

2020

Architecture of the centriole cartwheel‐containing region revealed by cryo‐electron tomography

Henning Paul-Julius Stahlberg, Paul Guichard, Virginie Hamel

Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture ...

2020

Imaging cellular ultrastructures using expansion microscopy (U-ExM)

Michaël Unser, Davide Gambarotto, Paul Guichard, Virginie Hamel, Denis Fortun

Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastruc ...

2019

Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails

Davide Demurtas, Paul Guichard, Virginie Hamel

Microtubule doublets (MTDs), consisting of an incomplete B-microtubule at the surface of a complete A-microtubule, provide a structural scaffold mediating intraflagellar transport and ciliary beating. Despite the fundamental role of MTDs, the molecular mec ...

AMER ASSOC ADVANCEMENT SCIENCE2019

Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis

Pierre Gönczy, Romain Hamelin, Florence Armand, Coralie Busso, Paul Guichard, Virginie Hamel, Isabelle Fluckiger

Centrioles are evolutionarily conserved macromolecular structures that are fundamental to form cilia, flagella, and centrosomes. Centrioles are 9-fold symmetrical microtubule-based cylindrical barrels comprising three regions that can be clearly distinguis ...

2017

Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

Henning Paul-Julius Stahlberg, Pierre Gönczy, Paul Guichard, Mircea Ioan Iacovache, Virginie Hamel, Niccolo Banterle, Isabelle Fluckiger, Veronika Nemcíková Villímová

How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-foldsymmetrical cartwheel structure typically 100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 pro ...

Springer Nature2017

SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture

Paul Guichard, Virginie Hamel

Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They are thought to assemble around a nine-fold symmetric cartwheel structure established by SAS-6 proteins. Here, we have engineered Chlamydomonas reinhardtii SAS- ...

Nature Publishing Group2016

Computational support for a scaffolding mechanism of centriole assembly

Pierre Gönczy, Paul Guichard, Virginie Hamel

Centrioles are essential for forming cilia, flagella and centrosomes. Successful centriole assembly requires proteins of the SAS-6 family, which can form oligomeric ring structures with ninefold symmetry in vitro. While important progress has been made in ...

2016

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