Publications by Arun Stephen Rajkumar

Mapping the fine structure of a eukaryotic promoter input-output function

Sebastian Maerkl, Nicolas Dénervaud, Arun Stephen Rajkumar

The precise tuning of gene expression levels is essential for the optimal performance of transcriptional regulatory networks. We created 209 variants of the Saccharomyces cerevisiae PHO5 promoter to quantify how different binding sites for the transcriptio ...

Nature Publishing Group2013

A chemostat array enables the spatio-temporal analysis of the yeast proteome

Michaël Unser, Felix Naef, Sebastian Maerkl, Nicolas Dénervaud, Ricard Delgado Gonzalo, Johannes Becker, Arun Stephen Rajkumar

Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min t ...

National Academy of Sciences2013

Systematic Analysis of Yeast Gene Expression by Synthetic Promoter Libraries

Arun Stephen Rajkumar

The precise tuning of gene expression levels is essential for optimal performance of transcriptional regulatory networks. Many regulatory components control promoter output, but transcription factor (TF) binding sites are amongst the most prominent. Beside ...

EPFL2013

Rapid Synthesis of Defined Eukaryotic Promoter Libraries

Sebastian Maerkl, Arun Stephen Rajkumar

Current gene synthesis methods allow the generation of long segments of dsDNA. We show that these techniques can be used to create synthetic regulatory elements and describe a method for the creation of completely defined, synthetic variants of the PHOS pr ...

Amer Chemical Soc2012