Person
Suliana Manley
Related publications (72)
Live-cell imaging powered by computation
The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...
Berlin2024Surface passivation and functionalisation for mass photometry
Suliana Manley, Jenny Sülzle, Laila Abdelaziz Abdelmoniem Elfeky
Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...
Wiley2024Chromosome organization shapes replisome dynamics in Caulobacter crescentus
Suliana Manley, Chen Zhang, Laurent Casini
DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replisomes has been observed in many bacterial specie ...
Nature Portfolio2024Nanoscale organization of the endogenous ASC speck
Suliana Manley, Christian Sieben
The NLRP3 inflammasome is a central component of the innate immune system. Its activation leads to for-mation of the ASC speck, a supramolecular assembly of the inflammasome adaptor protein ASC. Different models, based on ASC overexpression, have been prop ...
Cambridge2023Fluorescent D-Amino Acids for Super-resolution Microscopy of the Bacterial Cell Wall
Suliana Manley, Luc Reymond, Chen Zhang, Ophélie Rutschmann, Willi Leopold Stepp, Faustine Ambroisine Charline Ryckebusch, Juliette Griffie, Jiangtao Qiao
Fluorescent D-amino acids (FDAAs) have previ-ously been developed to enable in situ highlighting of locations of bacterial cell wall growth. Most bacterial cells lie at the edge of the diffraction limit of visible light; thus, resolving the precise details ...
AMER CHEMICAL SOC2022Event-driven acquisition for content-enriched microscopy
Suliana Manley, Martin Weigert, Chen Zhang, Willi Leopold Stepp, Dora Mahecic, Juliette Griffie
Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...
NATURE PORTFOLIO2022Optical microscopy gets down to angstroms
Suliana Manley, Giorgio Tortarolo
Super-resolution microscopy at angstrom precision could pave the way to optical structural biology in cells. ...
NATURE PORTFOLIO2022Nanoscale Pattern Extraction from Relative Positions of Sparse 3D Localizations
Suliana Manley, Christian Sieben
Inferring the organization of fluorescently labeled nanosized structures from single molecule localization microscopy (SMLM) data, typically obscured by stochastic noise and background, remains challenging. To overcome this, we developed a method to extrac ...
AMER CHEMICAL SOC2021Single-molecule localization microscopy
Suliana Manley, Juliette Griffie
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...
SPRINGERNATURE20213D Structure From 2D Microscopy Images Using Deep Learning
Suliana Manley, Christian Sieben
Understanding the structure of a protein complex is crucial in determining its function. However, retrieving accurate 3D structures from microscopy images is highly challenging, particularly as many imaging modalities are two-dimensional. Recent advances i ...
Lausanne2021