Carlotta Guiducci

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Selection of Structure-Switching DNA Aptamers Binding Soluble Small Molecules and SPR Validation of Enrichment

Carlotta Guiducci, Enrico Tenaglia, Fabio Mario Spiga

Humana Press2018

Comparison against current standards of a DNA aptamer for the label-free quantification of tobramycin in human sera employed for therapeutic drug monitoring

Carlotta Guiducci, Enrico Tenaglia, Thierry Buclin, Anna Ferretti

2018

Label-free identification of activated T lymphocytes through tridimensional microsensors on chip

Carlotta Guiducci, George Coukos, Enrico Tenaglia, Elena Bianchi, Enrica Rollo, Alexandre Harari

Label-free approaches to assess cell properties ideally suit the requirements of cell-based therapeutics, since they permit to characterize cells with minimal perturbation and manipulation, at the benefit of sample recovery and re-employment for treatment. ...

Elsevier2017

Metal-Coated SU-8 Structures for High-Density 3-D Microelectrode Arrays

Carlotta Guiducci, Samuel Kilchenmann, Pietro Maoddi, Enrica Rollo

Electric fields can be effectively used to sense, manipulate, and move particles in lab-on-a-chip devices. Nevertheless, the throughput of such devices is a critical issue, which can be effectively improved by increasing the height of the microchannels. Fo ...

Institute of Electrical and Electronics Engineers2016

Multi-Wire Tri-Gate Silicon Nanowires Reaching Milli-pH Unit Resolution in One Micron Square Footprint

Carlotta Guiducci, Enrico Accastelli, Paolo Scarbolo, Pierpaolo Palestri, Luca Selmi, Thomas Ernst

The signal-to-noise ratio of planar ISFET pH sensors deteriorates when reducing the area occupied by the device, thus hampering the scalability of on-chip analytical systems which detect the DNA polymerase through pH measurements. Top-down nano-sized tri-g ...

Mdpi Ag2016

Analysis of Dielectric Microbead Detection by Impedance Spectroscopy with Nanoribbons

Carlotta Guiducci, Enrico Accastelli, Paolo Scarbolo, Luca Selmi, Thomas Ernst

We present a quantitative numerical analysis of dielectric microbead detection experiments with nanoribbons operated in the AC small signal regime. To this purpose, a comprehensive model of nanoribbon operation in electrolyte environment and with DC/AC sig ...

Ieee2016

Label-Free Detection of Tobramycin in Serum by Transmission-Localized Surface Plasmon Resonance

Carlotta Guiducci, Thierry Buclin, Anna Ferretti, Giulia Cappi, Fabio Mario Spiga, Laurent Décosterd, Michael Beyeler

In order to improve the efficacy and safety of treatments, drug dosage needs to be adjusted to the actual needs of each patient in a truly personalized medicine approach. Key for widespread dosage adjustment is the availability of point-of-care devices abl ...

Amer Chemical Soc2015

More DNA–Aptamers for Small Drugs: A Capture–SELEX Coupled with Surface Plasmon Resonance and High-Throughput Sequencing

Carlotta Guiducci, Fabio Mario Spiga

To address limitations in the production of DNA aptamers against small molecules, we introduce a DNA-based capture-SELEX (systematic evolution of ligands by exponential enrichment) protocol with long and continuous randomized library for more flexibility, ...

ACS Publications2015

Post-cmos processing and 3d integration based on dry-film lithography

Carlotta Guiducci, Yusuf Leblebici, Yüksel Temiz

A method for performing a post processing pattern on a diced chip having a foot-print, comprises the steps of providing a support wafer; applying a first dry film photoresist to the support wafer; positioning a mask corresponding to the footprint of the di ...

2015

Hybridization chain reaction performed on a metal surface as a means of signal amplification in SPR and electrochemical biosensors

Carlotta Guiducci, Fabio Mario Spiga, Giampaolo Zuccheri

A more specific and intense signal is desirable for most kinds of biosensors for biomedical or environmental applications, and it is especially so for label-free biosensors. In this paper, we show that hybridization chain reaction (HCR) can be exploited fo ...

Elsevier2014

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