Publications by Liang Qiao | EPFL Graph Search

Detection of antimicrobial resistance-associated proteins by titanium dioxide-facilitated intact bacteria mass spectrometry

Hubert Girault, Horst Pick, Natalia Gasilova, Liang Qiao, Milica Jovic, Yingdi Zhu

Titanium dioxide-modified target plates were developed to enhance intact bacteria analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The plates were designed to photocatalytically destroy the bacterial envelope struct ...

2018

On-Chip Spyhole Nanoelectrospray Ionization Mass Spectrometry for Sensitive Biomarker Detection in Small Volumes

Hubert Girault, Xiaoqin Zhong, Liang Qiao, Géraldine Margaretha Alice Stauffer

A polyimide microfluidic chip with a microhole emitter (Ø 10–12 μm) created on top of a microchannel by scanning laser ablation has been designed for nanoelectrospray ionization (spyhole-nanoESI) to couple microfluidics with mass spectrometry. The spyhole- ...

2018

Maldi device and methods of preparation and use thereof

Hubert Girault, Horst Pick, Liang Qiao, Milica Jovic, Yingdi Zhu

A target plate for enhanced matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) analysis of intact biological entities. The target plate comprises an electrically conductive substrate (1) covered at least partially with immobilised pho ...

2018

Bacterial Whole Cell Typing by Mass Spectra Pattern Matching with Bootstrapping Assessment

Hubert Girault, Liang Qiao, Yu Lin

Bacterial typing is of great importance in clinical diagnosis, environmental monitoring, food safety analysis, and biological research. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is now widely used to analyze bacterial samples ...

American Chemical Society2017

Electrostatic spray ionization from 384-well microtiter plates for mass spectrometry analysis based enzyme assay and drug metabolism screening

Hubert Girault, Liang Qiao, Tzu-En Lin, Elena Tobolkina, Xiaoqin Zhong, Yan Deng, Emna Belghith

We have realized the direct ionization of samples from wells of microtiter plates under atmospheric conditions for mass spectrometry analysis without any liquid delivery system or any additional interface. The microtiter plate is a commercially available 3 ...

American Chemical Society2017

Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

Hubert Girault, Horst Pick, Niels Erik Lion, Natalia Gasilova, Michel Prudent, Liang Qiao, Yingdi Zhu, Alexandra Bondarenko

Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and ...

Royal Soc Chemistry2016

On-chip mesoporous functionalized magnetic microspheres for protein sequencing by extended bottom-up mass spectrometry

Hubert Girault, Yury Tsybin, Natalia Gasilova, Liang Qiao, Kristina Srzentic

Limited amount and extreme concentration variability of proteomic-related samples require efficient analyte preconcentration and purification prior to the mass spectrometry (MS)-based analysis. Preferably, these steps should be coupled on-line with chosen ...

American Chemical Society2016

Open channel-based microchip electrophoresis interfaced with mass spectrometry via electrostatic spray ionization

Hubert Girault, Natalia Gasilova, Liang Qiao, Yan Deng

The coupling between open channel-based microchip electrophoresis and mass spectrometry via electrostatic spray ionization is proposed for in situ detection of fractionated analytes. Electrophoretic separation is performed in an open channel fabricated in ...

Elsevier2016

Mass Barcode Signal Amplification for Multiplex Allergy Diagnosis by MALDI-MS

Hubert Girault, Natalia Gasilova, Liang Qiao, Xiaoqin Zhong

A highly sensitive method based on mass-barcoded gold nanoparticles (AuNPs) and immunomagnetic separation has been developed for multiplex allergy diagnosis by MALDI mass spectrometry in a component-resolved manner. Different analytical probes were prepare ...

American Chemical Society2016

Electrostatic Spray Ionization Method

Liang Qiao, Yu Lu, Elena Tobolkina, Romain Sartor

In an electrostatic spray ionization method for spraying a liquid layer from an insulating plate 2, the plate is arranged between two electrodes 1, 4. A constant high voltage power supply 3 is provided and an electric circuit is used to charge and discharg ...

2015