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The goal of our project (HES-SO n°11246) is to develop chemometric methods that enhance the development of chemical reactions. Semi-batch experiments are performed using a fully automated chemical reactor. Real-time analytical data, infrared (IR) spectra f ...
The stereospecific hydrolysis of phenylalanine esters by chymotrypsin can avantageously be undertaken in aq.-org. 2-phase systems. The partitioning of the deprotonated form of the substrate changes the pH characteristics of the reaction system drastically. ...
A classification methodology for the automatic detection of start- and endpoints of chemical and biotechnological reaction systems from spectral reaction data is proposed. In the calibration phase, several batch experiments must be conducted covering the e ...
L-Phenylalanine was produced from phenylalanine Pr esters by chymotrypsin in 2-phase systems (org. phase using iso-Bu Me ketone and aq. phase). Control of pH during the enzymic reaction was crit. The reaction was carried out in a batch fermentor or a fixed ...
A novel method for the detn. of partition coeffs. in aq.-org. two-phase systems is described. It is applicable for characterizing the distribution of substances undergoing proton dissocn. in the aq. phase. The method is based on titrn. by means of the org. ...
The development of a phasic system for the enzymic resoln. of racemic phenylalanine (I) up to benchtop pilot plant scale is described. The principle is based upon the hydrolysis of racemic Pr ester of I with immobilized a-chymotrypsin (II) in which only th ...
