Romain Guiet

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T Cell-Mediated Transport of Polymer Nanoparticles across the Blood-Brain Barrier

Harm-Anton Klok, Olivier Burri, Arne Seitz, Romain Guiet, Maxime Ayer, Markus Schuster

Delivery of therapeutics to the central nervous system (CNS) is challenging due to the presence of the blood-brain barrier (BBB). Amongst various approaches that have been explored to facilitate drug delivery to the CNS, the use of cells that have the intr ...

WILEY2020

Hookworms Evade Host Immunity by Secreting a Deoxyribonuclease to Degrade Neutrophil Extracellular Traps

Arne Seitz, Romain Guiet, Nicola Harris, Beatrice Volpe, Tiffany Babette Angélique Bouchery, Kathleen Shah, Mati André Marie Moyat, Manuel Kulagin

Hookworms cause a major neglected tropical disease, occurring after larvae penetrate the host skin. Neutrophils are phagocytes that kill large pathogens by releasing neutrophil extracellular traps (NETs), but whether they target hookworms during skin infec ...

CELL PRESS2020

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Claudia Battistella, Romain Guiet

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

American Chemical Society2019

A Derived Positional Mapping of Inhibitory Subtypes in the Somatosensory Cortex

Henry Markram, Olivier Burri, Romain Guiet, Julie Meystre, Felix Schürmann, Daniel Keller, Rahul Valiya Veettil

Obtaining a catalog of cell types is a fundamental building block for understanding the brain. The ideal classification of cell-types is based on the profile of molecules expressed by a cell, in particular, the profile of genes expressed. One strategy is, ...

2019

Podosomes, But not the Maturation status, Determine the Protease-Dependent 3D Migration in human Dendritic cells

Romain Guiet

Dendritic cells (DC) are professional Antigen-Presenting Cells scattered throughout antigen-exposed tissues and draining lymph nodes, and survey the body for pathogens. Their ability to migrate through tissues, a 3D environment, is essential for an effecti ...

2018

Applying Whole Organ Clearing Techniques To Spinal Cord Injury: A Survey Of Techniques

Grégoire Courtine, Thierry Laroche, Olivier Burri, Quentin Barraud, Romain Guiet, Laura Battista, Newton Cho

MARY ANN LIEBERT2018

Antibody-Mediated Trapping of Helminth Larvae Requires CD11b and Fc gamma Receptor I

Arne Seitz, Romain Guiet, Duncan-Bruce Sutherland, Nicola Harris, Benjamin John Marsland, Beatrice Volpe, Julia Esser-von Bieren, Manuel Kulagin

Infections with intestinal helminths severely impact on human and veterinary health, particularly through the damage that these large parasites inflict when migrating through host tissues. Host immunity often targets the motility of tissue-migrating helmin ...

American Association of Immunologists ; Oxford University Press2015

Correlative multicolor 3D SIM and STORM microscopy

Pierre Gönczy, Arne Seitz, Paul Guichard, Romain Guiet, Virginie Hamel, Isabelle Fluckiger, Mathias Fournier

Within the last decade, super-resolution methods that surpass the diffraction limit of light microscopy have provided invaluable insight into a variety of biological questions. Each of these approaches has inherent advantages and limitations, such that the ...

Optical Soc Amer2014

Antibodies Trap Tissue Migrating Helminth Larvae and Prevent Tissue Damage by Driving IL-4Rα-Independent Alternative Differentiation of Macrophages

Arne Seitz, Romain Guiet, Nicola Harris, Beatrice Volpe, Alessandra Piersigilli, Julia Esser-von Bieren, Ilaria Mosconi, Fei Chen

Approximately one-third of the world's population suffers from chronic helminth infections with no effective vaccines currently available. Antibodies and alternatively activated macrophages (AAM) form crucial components of protective immunity against chall ...

Public Library of Science2013

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Antibodies Trap Tissue Migrating Helminth Larvae and Prevent Tissue Damage by Driving IL-4Rα-Independent Alternative Differentiation of Macrophages

Arne Seitz, Romain Guiet, Nicola Harris, Beatrice Volpe, Alessandra Piersigilli, Julia Esser-von Bieren, Ilaria Mosconi, Fei Chen

Public Library of Science2013