Person
Vladimir Kopysov
This person is no longer with EPFL
Related publications (18)
Gentle nano-electrospray ion source for reliable and efficient generation of microsolvated ions
Vladimir Kopysov, Andrei Zviagin
We present herein the design of a nano-electrospray ion source capable of reliable generation of large quantities of microsolvated ions. The source is based on a triple molecular skimmer scheme and can be quickly tuned to generate bare ions or their ionic ...
AIP Publishing2022Spectroscopic Evidence for Peptide-Bond-Selective Ultraviolet Photodissociation
Vladimir Kopysov, Aleksandr Pereverzev
We study the photodissociation induced by ultraviolet excitation of amide bonds in gas-phase protonated peptides. Jointly, mass spectrometry and cold ion spectroscopy provide evidence for a selective non-statistical dissociation of specific peptide bonds i ...
2020Method for Identification of Threonine Isoforms in Peptides by Ultraviolet Photofragmentation of Cold Ions
Vladimir Kopysov, Aleksandr Pereverzev, Elizaveta Solovyeva
Identification of isomeric amino acid residues in peptides and proteins is challenging but often highly desired in proteomics. One of the practically important cases that require isomeric assignments is that associated with single-nucleotide polymorphism s ...
AMER CHEMICAL SOC2019Interplay of H-Bonds with Aromatics in Isolated Complexes Identifies Isomeric Carbohydrates
Vladimir Kopysov, Ruslan Yamaletdinov, Aleksandr Pereverzev
The tremendous isomeric diversity of carbohydrates enables a wide range of their biological functions but makes the identification and study of these molecules difficult. We investigated the ability of intermolecular interactions to communicate structural ...
WILEY-V C H VERLAG GMBH2019Gas-phase structures reflect the pain-relief potency of enkephalin peptides
Vladimir Kopysov, Aleksandr Pereverzev
We use cold ion spectroscopy and quantum-chemical computations to solve the structures of opioid peptides enkephalins in the gas phase. The derived structural parameters clearly correlate with the known pharmacological efficiency of the studied drugs, sugg ...
2019Peptide Bond Ultraviolet Absorption Enables Vibrational Cold-Ion Spectroscopy of Nonaromatic Peptides
Vladimir Kopysov, Aleksandr Pereverzev
Peptide-bond VUV absorption is inherent to all proteins and peptides. Although widely exploited in top-down proteomics for photodissociation, this absorption has never been spectroscopically characterized in the gas phase. We have measured VUV/UV photofrag ...
2018Initial Steps of Amyloidogenic Peptide Assembly Revealed by Cold-Ion Spectroscopy
Vladimir Kopysov, Natalia Nagornova
The early stages of fibril formation are difficult to capture in solution. We use cold-ion spectroscopy to examine an 11-residue peptide derived from the protein transthyretin and clusters of this fibre-forming peptide containing up to five units in the ga ...
Wiley-Blackwell2018Intrinsic structure of pentapeptide Leu-enkephalin: geometry optimization and validation by comparison of VSCF-PT2 calculations with cold ion spectroscopy
Vladimir Kopysov, Oleg Boyarkine, Aleksandr Pereverzev, Robert Benny Gerber
The intrinsic structure of an opioid peptide [Ala2, Leu5]-leucine enkephalin (ALE) has been investigated using first-principles based vibrational self-consistent field (VSCF) theory and cold ion spectroscopy. IR-UV double resonance spectroscopy revealed th ...
2018Identification of Isomeric Ephedrines by Cold Ion UV Spectroscopy: Toward Practical Implementation
Ephedrine and pseudoephedrine are stimulant drugs whose use is prohibited in athletic competition by the World Anti Doping Agency (WADA) at very different threshold doping violation concentrations. We use a recently developed universal approach that integr ...
2017High Susceptibility of Histidine to Charge Solvation Revealed by Cold Ion Spectroscopy
Vladimir Kopysov, Aleksandr Pereverzev
Histidine remained the last aromatic amino acid for which the intrinsic spectroscopic properties and structures were obscure. We measured the UV and IR spectra of protonated histidine, isolated in the gas phase, using photofragmentation cold ion spectrosco ...
Wiley-Blackwell2017