Person
Daniel Migliozzi
Related publications (17)
Multi-well plate lid for single-step pooling of 96 samples for high-throughput barcode-based sequencing
Bart Deplancke, Daniel Migliozzi, Gilles Weder, Riccardo Dainese, Daniel Alpern, Hüseyin Baris Atakan, Mustafa Demir, Dariia Gudkova
High-throughput transcriptomics is of increasing fundamental biological and clinical interest. The generation of molecular data from large collections of samples, such as biobanks and drug libraries, is boosting the development of new biomarkers and treatm ...
Dordrecht2024Aperture-Controlled Fabrication of All-Dielectric Structural Color Pixels
Olivier Martin, Philippe Renaud, Arnaud Bertsch, Daniel Migliozzi, Evgenii Glushkov, Clémentine Sophie Sarah Lipp, Hsiang-Chu Wang, Audrey Zoé Marie Jacquillat
While interference colors have been known for a longtime, conventionalcolor filters have large spatial dimensions and cannot be used tocreate compact pixelized color pictures. Here we report a simple yetelegant interference-based method of creating microsc ...
AMER CHEMICAL SOC2023Phase calibration of a basic bright-field microscope for 3D metrology of transparent samples at the nanoscale
Martinus Gijs, Daniel Migliozzi, Bingying Zhao
Optical retrieval of the structure of transparent objects at the nano-scale requires adapted methods capable of probing their interaction with suitable light. Scattering events, for instance, depend on the content and the arrangement of the medium encounte ...
SPIE-INT SOC OPTICAL ENGINEERING2021Planar hydrodynamic traps and buried channels for bead and cell trapping and releasing
Philippe Renaud, Arnaud Bertsch, Daniel Migliozzi, Clémentine Sophie Sarah Lipp, Jonathan Bastien Cottet
We present a novel concept for the controlled trapping and releasing of beads and cells in a PDMS microfluidic channel without obstacles present around the particle or in the channel. The trapping principle relies on a two-level microfluidic configuration: ...
ROYAL SOC CHEMISTRY20213D nanometrology of transparent objects by phase calibration of a basic bright-field microscope for multiple illumination apertures
Martinus Gijs, Daniel Migliozzi, Bingying Zhao
Optical retrieval of the structure of transparent objects at the nanoscale requires adapted techniques capable of probing their interaction with light. Here, we considered a method based on calibration of the defocusing with partially coherent illumination ...
2020Structural transitions and mechanochemical coupling in the nucleoprotein filament explain homology selectivity and Rad51 protein cooperativity in cellular DNA repair
The nucleoprotein filament (NPF) is the fundamental element of homologous recombination (HR), a major mechanism for the repair of double-strand DNA breaks in the cell. The NPF is made of the damaged DNA strand surrounded by recombinase proteins, and its se ...
AMER PHYSICAL SOC2020Integration of microfluidics and multimodal optics for translational bioanalytics
Numerous analytical techniques for life sciences have been at the basis of the improvement of medical diagnostics and treatments. Current challenges in bioanalytics are related to the conception of methods enabling large amount of information to be extract ...
EPFL2019Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections
Martinus Gijs, Daniel Migliozzi, Diego Gabriel Dupouy, Benjamin Pelz
Because of the close interaction between tumors and the immune system, immunotherapies are nowadays considered as the most promising treatment against cancer. In order to define the diagnosis and the subsequent therapy, crucial information about the immune ...
2019Combining fluorescence-based image segmentation and automated microfluidics for ultrafast cell-by-cell assessment of biomarkers for HER2-type breast carcinoma
Martinus Gijs, Daniel Migliozzi, Huu Tuan Nguyen
Immunohistochemistry (IHC) is one of the main clinical techniques for biomarker assessment on tissue biopsies. It consists in chromogenic labeling with specific antibodies, followed by optical imaging, and it is used for diagnosis and therapeutic targeting ...
SPIE-Intl Soc Optical Eng2019High-content, cell-by-cell assessment of HER2 overexpression and amplification: a tool for intratumoral heterogeneity detection in breast cancer
Martinus Gijs, Daniel Migliozzi, Huu Tuan Nguyen
Immunohistochemistry and fluorescence in situ hybridization are the two standard methods for human epidermal growth factor receptor 2 (HER2) assessment. However, they have severe limitations to assess quantitatively intratumoral heterogeneity (ITH) when mu ...
NATURE PUBLISHING GROUP2019