Publications by Johannes Julius Bues

Engineering next-generation microfluidic technologies for single-cell phenomics

Bart Deplancke, Johannes Julius Bues, Guido Van Mierlo, Camille Lucie Germaine Lambert, Orane Josiane Guillaume-Gentil

Springer Science and Business Media LLC2025

The evolution of DNA sequencing with microfluidics

Bart Deplancke, Johannes Julius Bues, Guido Van Mierlo, Camille Lucie Germaine Lambert

The adoption of microfluidics was fundamental to the development of cost-effective, high-throughput DNA sequencing. As the field progresses towards multi-omics, we reflect on the key concepts underlying microfluidics and how resulting engineering advances ...

NATURE PORTFOLIO2024

System and method for single cell phenotypical profiling and deterministic nanoliter-droplet encapsulation and deterministic droplet consortia assemblies

Bart Deplancke, Jörn Pezoldt, Johannes Julius Bues

The present invention concerns a system for phenotypical profiling of at least one object and deterministic nanoliter-droplet encapsulation, comprising sample supplying means, buffer supplying means; a microfluidic chip comprising an encapsulation area or ...

2023

Deterministic scRNA-seq captures variation in intestinal crypt and organoid composition

Matthias Lütolf, Bart Deplancke, Esther Amstad, Vincent Roland Julien Gardeux, Julie Marie Russeil, Rita Sarkis, Jörn Pezoldt, Johannes Julius Bues, Antonius Chrisnandy, Riccardo Dainese

DisCo is a deterministic droplet microfluidics tool for single-cell analysis on low cell input samples, which is demonstrated to profile individual intestinal organoids and in vivo-derived small tissues. ...

NATURE PORTFOLIO2022

Towards scalable and deterministic single cell gene expression profiling

Johannes Julius Bues

Characterizing tissue composition at the cellular level is essential for understanding the human body in health and disease, and has been one of the frontiers in biology for centuries. Recently developed single-cell RNA-sequencing (scRNA-seq) techniques no ...

EPFL2021

Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip

Bart Deplancke, Esther Amstad, Johannes Julius Bues, Riccardo Dainese, Marjan Biocanin

Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution. One of the catalyzing technologies is single cell droplet microfluidics, ...

2019

Device for high throughput single-cell studies

Bart Deplancke, Johannes Julius Bues, Riccardo Dainese, Marjan Biocanin

The present invention concerns a microfluidic device or chip including at least one inlet for introducing at least one object into the device; an oil inlet for introducing an oil that supports droplet formation into the device or a droplet forming substanc ...

2018

Dissecting the brown adipogenic regulatory network using integrative genomics

Bart Deplancke, Wanze Chen, Vincent Roland Julien Gardeux, Julie Marie Russeil, Johannes Julius Bues, Daniel Alpern, Sunil Kumar Raghav, Petra Catalina Schwalie, Rachana Narendra Pradhan

Brown adipocytes regulate energy expenditure via mitochondrial uncoupling, which makes them attractive therapeutic targets to tackle obesity. However, the regulatory mechanisms underlying brown adipogenesis are still poorly understood. To address this, we ...

Springer Nature2017