Publications by Niccolo Banterle

Grand canonical Brownian dynamics simulations of adsorption and self-assembly of SAS-6 rings on a surface

The Spindle Assembly Abnormal Protein 6 (SAS-6) forms dimers, which then self-assemble into rings that are critical for the nine-fold symmetry of the centriole organelle. It has recently been shown experimentally that the self-assembly of SAS-6 rings is st ...

AIP Publishing2023

Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture

Pierre Gönczy, Georg Fantner, Georgios Hatzopoulos, Adrian Pascal Nievergelt, Niccolo Banterle, Santiago Harald Andany, Charlène Cécile Geneviève Brillard

Discovering mechanisms governing organelle assembly is a fundamental pursuit in biology. The centriole is an evolutionarily conserved organelle with a signature 9-fold symmetrical chiral arrangement of microtubules imparted onto the cilium it templates. Th ...

Nature Portfolio2021

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly

Pierre Gönczy, Georg Fantner, Georgios Hatzopoulos, Tatiana Favez, Oliver Hantschel, Virginie Hamel, Niccolo Banterle, Santiago Harald Andany

Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that sca ...

Nature Research2021

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Khalid Ashraf Mohie Ibrahim, Davide Gambarotto, Paul Guichard, Virginie Hamel, Niccolo Banterle, Dora Mahecic, Denis Fortun

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

2020

Multicolor Single-Particle Reconstruction of Protein Complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

CELL PRESS2019

High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6

Pierre Gönczy, Adrian Pascal Nievergelt, Georg Fantner, Niccolo Banterle, Santiago Harald Andany

The self-assembly of protein complexes is at the core of many fundamental biological processes1, ranging from the polymerization of cytoskeletal elements, such as microtubules2, to viral capsid formation and organelle assembly3. To reach a comprehensive un ...

2018

Reconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy

Michaël Unser, Pierre Gönczy, Paul Guichard, Virginie Hamel, Denis Fortun, Niccolo Banterle

Institute of Electrical and Electronics Engineers2018

Multicolor single-particle reconstruction of protein complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

2018

Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

Henning Paul-Julius Stahlberg, Pierre Gönczy, Niccolo Banterle, Paul Guichard, Mircea Ioan Iacovache, Virginie Hamel, Isabelle Fluckiger, Veronika Nemcíková Villímová

How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-foldsymmetrical cartwheel structure typically 100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 pro ...

Springer Nature2017

Centriole Biogenesis: From Identifying the Characters to Understanding the Plot

Pierre Gönczy, Niccolo Banterle

The centriole is a beautiful microtubule-based organelle that is critical for the proper execution of many fundamental cellular processes, including polarity, motility, and division. Centriole biogenesis, the making of this miniature architectural wonder, ...

Annual Reviews2017