Person
Andrew Charles Oates
Related publications (99)
A Robotic Surgery Platform for Automated Tissue Micromanipulation in Zebrafish Embryos
Andrew Charles Oates, Mahmut Selman Sakar, Ece Özelçi, Laurel Ann Rohde, Erfan Etesami
Microsurgical manipulations are key experimental techniques in life science research, particularly in embryology. These techniques are most often performed manually by highly skilled scientists, posing limitations on speed, precision, and reproducibility. ...
2023Reaction wavefront theory of notochord segment patterning
The vertebrate axis is segmented into repetitive structures, the vertebrae. In fish, these segmented structures are thought to form from the paraxial mesoderm and the adjacent notochord. Recent work revealed an autonomous patterning mechanism in the zebraf ...
FRONTIERS MEDIA SA2022Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation
Andrew Charles Oates, Mahmut Selman Sakar, Ece Özelçi, Erik Mailand, Matthias Rüegg
Classic microsurgical techniques, such as those used in the early 1900s by Mangold and Spemann, have been instrumental in advancing our understanding of embryonic development. However, these techniques are highly specialized, leading to issues of inter-ope ...
2022Preparation of Single-Somite Explants from Zebrafish Embryos
Andrew Charles Oates, Sundar Ram Naganathan
The body axis of vertebrate embryos is periodically subdivided into 3D multicellular units called somites. While genetic oscillations and molecular prepatterns determine the initial length-scale of somites, mechanical processes have been implicated in sett ...
JOURNAL OF VISUALIZED EXPERIMENTS2022Theory of time delayed genetic oscillations with external noisy regulation
Andrew Charles Oates, Laurel Ann Rohde, Jose Negrete Jr
Video Abstract Video Abstract: Theory of time delayed genetic oscillations with external noisy regulation We present a general theory of noisy genetic oscillators with externally regulated production rate and multiplicative noise. The observables that char ...
IOP PUBLISHING LTD2021From local resynchronization to global pattern recovery in the zebrafish segmentation clock
Integrity of rhythmic spatial gene expression patterns in the vertebrate segmentation clock requires local synchronization between neighboring cells by Delta-Notch signaling and its inhibition causes defective segment boundaries. Whether deformation of the ...
ELIFE SCIENCES PUBLICATIONS LTD2021Patterning and mechanics of somite boundaries in zebrafish embryos
Andrew Charles Oates, Sundar Ram Naganathan
The body axis of vertebrates is subdivided into repetitive compartments called somites, which give rise primarily to the segmented architecture of the musculoskeletal system in the adult body. Somites form in a sequential and rhythmic manner in embryos and ...
2020What are you synching about? Emerging complexity of Notch signaling in the segmentation clock
Andrew Charles Oates, Olivier François Venzin
The Segmentation clock is a population of cellular genetic oscillators, located in the posterior of the elongating vertebrate embryo, that governs the rhythmic and sequential segmentation of the body axis into somites. Somites are blocks of cells that give ...
ACADEMIC PRESS INC ELSEVIER SCIENCE2020Waiting on the Fringe: cell autonomy and signaling delays in segmentation clocks
The rhythmic and sequential segmentation of the vertebrate body axis into somites during embryogenesis is governed by a multicellular, oscillatory patterning system called the segmentation clock. Despite many overt similarities between vertebrates, differe ...
2020Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
Andrew Charles Oates, Rachna Narayanan
In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non- ...
BIO-PROTOCOL2019