Publications by Willi Leopold Stepp

Mitochondrial Structure, Dynamics, and Physiology Light Microscopy to Disentangle the Network

Suliana Manley, Willi Leopold Stepp, Julius Hans Bernd Winter, Juan Cruz Landoni Martin, Tatjana Isabelle Kleele

Mitochondria serve as energetic and signaling hubs of the cell: This function results from the complex interplay between their structure, function, dynamics, interactions, and molecular organization. The ability to observe and quantify these properties oft ...

ANNUAL REVIEWS2024

Polyphosphate affects cytoplasmic and chromosomal dynamics in nitrogen-starved Pseudomonas aeruginosa

Suliana Manley, Willi Leopold Stepp, Sofia Magkiriadou

Polyphosphate (polyP) synthesis is a ubiquitous stress and starvation response in bacteria. In diverse species, mutants unable to make polyP have a wide variety of physiological defects, but the mechanisms by which this simple polyanion exerts its effects ...

National Academy of Sciences2024

Event-driven acquisition for content-enriched microscopy

Suliana Manley, Willi Leopold Stepp, Martin Weigert, Dora Mahecic, Chen Zhang, Juliette Griffie

CELL PRESS2023

Event-driven acquisition for content-enriched microscopy

Suliana Manley, Dora Mahecic, Willi Leopold Stepp, Chen Zhang, Martin Weigert, Juliette Griffie

Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...

2022

Fluorescent D-Amino Acids for Super-resolution Microscopy of the Bacterial Cell Wall

Suliana Manley, Luc Reymond, Ophélie Rutschmann, Willi Leopold Stepp, Juliette Griffie, Chen Zhang, Jiangtao Qiao, Faustine Ambroisine Charline Ryckebusch

Fluorescent D-amino acids (FDAAs) have previ-ously been developed to enable in situ highlighting of locations of bacterial cell wall growth. Most bacterial cells lie at the edge of the diffraction limit of visible light; thus, resolving the precise details ...

2022