Publication
Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L of recombinant protein have been obtained in stirred bioreactors. Modified alphaviruses have provided a fast and efficient expression technology based on viral vectors.
David Hacker, Sowmya Balasubramanian
Florian Maria Wurm, David Hacker, Sowmya Balasubramanian
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Virginie Bachmann, Xiao Shen