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Production of recombinant protein therapeutics in cultivated mammalian cells

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Production of recombinant protein therapeutics in cultivated mammalian cells

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Use of Orbital Shaken Disposable Bioreactors for Mammalian Cell Cultures from the Milliliter-Scale to the 1,000-Liter Scale

Development of high-titer transient gene expression processes for manufacturing recombinant proteins

Accelerating the development time of recombinant mammalian cell lines producing high titers of protein therapeutics

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester

Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives

O6-alkylguanine-DNA alkyltransferase as a new tool for functional proteomics

An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells