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Simulations of electrophoretic collisions of DNA knots with gel obstacles

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Untangling the Mechanics and Topology in the Frictional Response of Long Overhand Elastic Knots

Pedro Miguel Nunes Pereira de Almeida Reis

We combine experiments and theory to study the mechanics of overhand knots in slender elastic rods under tension. The equilibrium shape of the knot is governed by an interplay between topology, friction, and bending. We use precision model experiments to q ...

American Physical Society2015

New analytical tools combining gel electrophoresis and mass spectrometry

Elena Tobolkina

Proteomics has been one of the main projects challenging biological and analytical chemists for many years. The separation, identification and quantification of all the proteins expressed within biological systems remain the main objectives of proteomics. ...

EPFL2014

AC Magnetoelectrical Susceptibility Study of Multiferroics

Abhisek Datta

Multiferroics are materials in which ferroelectric and magnetic e.g., ferromagnetic or antiferromagnetic orders co-exist. Some of these show the magnetoelectric effect which is the coupling between electric and magnetic degrees of freedom leading to induct ...

2014

Sedimentation of macroscopic rigid knots and its relation to gel electrophoretic mobility of DNA knots

Giovanni Dietler, Cédric Weber, Andrzej Stasiak, Mathias Carlen

We address the general question of the extent to which the hydrodynamic behaviour of microscopic freely fluctuating objects can be reproduced by macrosopic rigid objects. In particular, we compare the sedimentation speeds of knotted DNA molecules undergoin ...

Nature Publishing Group2013

Fourier Approximation Of Symmetric Ideal Knots

Henryk Gerlach, Mathias Carlen

Enforcing a specific symmetry group on a curve, knotted or not, is not trivial using standard interpolations such as polygons or splines. For a prescribed symmetry group we present a symmetrization process based on a Fourier description of a knot. The pres ...

2012

Tightening of DNA knots by supercoiling facilitates their unknotting by type II DNA topoisomerases

Giovanni Dietler, Andrzej Stasiak, Guillaume Witz

Using numerical simulations, we compare properties of knotted DNA molecules that are either torsionally relaxed or supercoiled. We observe that DNA supercoiling tightens knotted portions of DNA molecules and accentuates the difference in curvature between ...

2011

DNA Topology

Guillaume Witz

This thesis explores different aspects of DNA topology through experimental and numerical techniques. Topology is a vast mathematical field, that deals with the spatial properties of objects undergoing continuous deformations, but here it is restricted to ...

EPFL2010

Computation and visualization of ideal knot shapes

Mathias Carlen

We investigate numerical simulations and visualizations of the problem of tying a knot in a piece of rope. The goal is to use the least possible rope of a fixed, prescribed radius to tie a particular knot, e.g. a trefoil, a figure eight, and so on. The rop ...

EPFL2010

DNA supercoiling and its role in DNA decatenation and unknotting

Andrzej Stasiak, Guillaume Witz

Chromosomal and plasmid DNA molecules in bacterial cells are maintained under torsional tension and are therefore supercoiled. With the exception of extreme thermophiles, supercoiling has a negative sign, which means that the torsional tension diminishes t ...

Oxford University Press2010

Ideal knots and other packing problems of tubes

Henryk Gerlach

This thesis concerns optimal packing problems of tubes, or thick curves, where thickness is defined as follows. Three points on a closed space curve define a circle. Taking the infimum over all radii of pairwise-distinct point triples defines the thickness ...

EPFL2010