An efficient strategy for visualizing human fingerprints on a poly(vinyldene difluoride) membrane (PVDF) by sanning electrochemical microscopy (SECM) has been developed. Compared to a classical ink fingerprint image, here the ink is replaced by an aqueous solution of bovine serum albumin (BSA). After placing the ‘‘inked’’ finger on a PVDF membrane, the latent image is stained by silver nitrate and the fingerprint is imaged electrochemically using potassium hexachloroiridate (III) (K3IrCl6) as a redox mediator. SECM images with an area of 5 mm · 3 mm have been recorded with a high-resolution using a 25-lm-diameter Pt disk-shaped microelectrode. Pores in the skin (40–120 lm in diameter) and relative locations of ridges were clearly observed. The factors relevant to the quality of fingerprint images are discussed.