Protein purificationProtein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the specification of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins.
Proof calculusIn mathematical logic, a proof calculus or a proof system is built to prove statements. A proof system includes the components: Language: The set L of formulas admitted by the system, for example, propositional logic or first-order logic. Rules of inference: List of rules that can be employed to prove theorems from axioms and theorems. Axioms: Formulas in L assumed to be valid. All theorems are derived from axioms. Usually a given proof calculus encompasses more than a single particular formal system, since many proof calculi are under-determined and can be used for radically different logics.
Proof of workProof of work (PoW) is a form of cryptographic proof in which one party (the prover) proves to others (the verifiers) that a certain amount of a specific computational effort has been expended. Verifiers can subsequently confirm this expenditure with minimal effort on their part. The concept was invented by Moni Naor and Cynthia Dwork in 1993 as a way to deter denial-of-service attacks and other service abuses such as spam on a network by requiring some work from a service requester, usually meaning processing time by a computer.
Formal proofIn logic and mathematics, a formal proof or derivation is a finite sequence of sentences (called well-formed formulas in the case of a formal language), each of which is an axiom, an assumption, or follows from the preceding sentences in the sequence by a rule of inference. It differs from a natural language argument in that it is rigorous, unambiguous and mechanically verifiable. If the set of assumptions is empty, then the last sentence in a formal proof is called a theorem of the formal system.
Constructive proofIn mathematics, a constructive proof is a method of proof that demonstrates the existence of a mathematical object by creating or providing a method for creating the object. This is in contrast to a non-constructive proof (also known as an existence proof or pure existence theorem), which proves the existence of a particular kind of object without providing an example. For avoiding confusion with the stronger concept that follows, such a constructive proof is sometimes called an effective proof.
Facilitated diffusionFacilitated diffusion (also known as facilitated transport or passive-mediated transport) is the process of spontaneous passive transport (as opposed to active transport) of molecules or ions across a biological membrane via specific transmembrane integral proteins. Being passive, facilitated transport does not directly require chemical energy from ATP hydrolysis in the transport step itself; rather, molecules and ions move down their concentration gradient reflecting its diffusive nature.
Transmembrane proteinA transmembrane protein (TP) is a type of integral membrane protein that spans the entirety of the cell membrane. Many transmembrane proteins function as gateways to permit the transport of specific substances across the membrane. They frequently undergo significant conformational changes to move a substance through the membrane. They are usually highly hydrophobic and aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.
