Publication
We have purified a 100 kDa protein, resolved in a Southwestern binding screen of total nuclear proteins from Hela cells with double-stranded human telomeric probe. A polyclonal antiserum raised by this protein recognizes purified nucleolin and stains nucleoli in growing Hela cells. We demonstrate that a truncated form of human nucleolin and a purified deletion derivative of mouse nucleolin bind in vitro to duplex telomeric DNA. This study suggests a new link between telomeres and the nucleolus.
David Lyndon Emsley, Pierrick Berruyer, Andrea Bertarello, Baptiste Busi
Francesco Stellacci, Luciano Andres Abriata, Lixia Wei, Suiyang Liao