Solar timeSolar time is a calculation of the passage of time based on the position of the Sun in the sky. The fundamental unit of solar time is the day, based on the synodic rotation period. Traditionally, there are three types of time reckoning based on astronomical observations: apparent solar time and mean solar time (discussed in this article), and sidereal time, which is based on the apparent motions of stars other than the Sun. A tall pole vertically fixed in the ground casts a shadow on any sunny day.
Ultraviolet–visible spectroscopyUV spectroscopy or UV–visible spectrophotometry (UV–Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in part of the ultraviolet and the full, adjacent visible regions of the electromagnetic spectrum. Being relatively inexpensive and easily implemented, this methodology is widely used in diverse applied and fundamental applications. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy.
Hoechst stainHoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation–emission spectra.
Universal TimeUniversal Time (UT or UT1) is a time standard based on Earth's rotation. While originally it was mean solar time at 0° longitude, precise measurements of the Sun are difficult. Therefore, UT1 is computed from a measure of the Earth's angle with respect to the International Celestial Reference Frame (ICRF), called the Earth Rotation Angle (ERA, which serves as a modern replacement for Greenwich Mean Sidereal Time). UT1 is the same everywhere on Earth. UT1 is required to follow the relationship ERA = 2π(0.
Sodium fluorideSodium fluoride (NaF) is an inorganic compound with the formula . It is a colorless or white solid that is readily soluble in water. It is used in trace amounts in the fluoridation of drinking water to prevent tooth decay, and in toothpastes and topical pharmaceuticals for the same purpose. In 2020, it was the 265th most commonly prescribed medication in the United States, with more than 1 million prescriptions. It is also used in metallurgy and in medical imaging.
Bicarbonate buffer systemThe bicarbonate buffer system is an acid-base homeostatic mechanism involving the balance of carbonic acid (H2CO3), bicarbonate ion (HCO), and carbon dioxide (CO2) in order to maintain pH in the blood and duodenum, among other tissues, to support proper metabolic function. Catalyzed by carbonic anhydrase, carbon dioxide (CO2) reacts with water (H2O) to form carbonic acid (H2CO3), which in turn rapidly dissociates to form a bicarbonate ion (HCO ) and a hydrogen ion (H+) as shown in the following reaction: As with any buffer system, the pH is balanced by the presence of both a weak acid (for example, H2CO3) and its conjugate base (for example, HCO) so that any excess acid or base introduced to the system is neutralized.
Terrestrial TimeTerrestrial Time (TT) is a modern astronomical time standard defined by the International Astronomical Union, primarily for time-measurements of astronomical observations made from the surface of Earth. For example, the Astronomical Almanac uses TT for its tables of positions (ephemerides) of the Sun, Moon and planets as seen from Earth. In this role, TT continues Terrestrial Dynamical Time (TDT or TD), which succeeded ephemeris time (ET). TT shares the original purpose for which ET was designed, to be free of the irregularities in the rotation of Earth.
Unix timeCurrent Unix time () Unix time is a date and time representation widely used in computing. It measures time by the number of seconds that have elapsed since 00:00:00 UTC on 1 January 1970, the Unix epoch, without adjustments made due to leap seconds. In modern computing, values are sometimes stored with higher granularity, such as microseconds or nanoseconds. Unix time originated as the system time of Unix operating systems. It has come to be widely used in other computer operating systems, , programming languages, and databases.
