Interface Layering Phenomena in Capacitance Detection of DNA with Biochips
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This work presents a simple microfluidic device with an integrated thin-film heater for studies of DNA hybridization kinetics and double-stranded DNA melting temperature measurements. The heating characteristics of the device were evaluated with a novel, n ...
NOVELTY - The micro-array device has several micro-locations (3) associated with a preset DNA sequence. Several micro-sensors are positioned corresponding to the micro-locations. The micro-sensors output electrical signals indicating the absorption of ultr ...
Univ Alma Mater Studiorum Di Bologna; Ricco B; Guiducci C2006
This paper describes a heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence (HECL) of a rhodamine label. Thin oxide-film coated aluminum and silicon electrodes were modified with an aminosilane layer and ...
This paper describes the design of a smart sensor for label-free detection of DNA hybridization. The sensor is based on a direct electrical transduction principle: it measures impedance variation at the interface between a bio-functionalized electrode and ...
Quantitative PCR (qPCR) is a powerful technique that is now commonly used in many research and clinical laboratories. Although it allows precise quantification of specific DNA sequences, it is often not used at its full potential. A number of data collecti ...
In this work we present the first results on a 'label-free' DNA analysis system based on hydrogenated amorphous silicon (a-Si:H) thin film sensors. The detection mechanism is based on absorbance measurements performed on DNA molecules in a buffer solution. ...
We developed standard curves based on plasmids containing a 16S rRNA gene of a member of one of the three genera Dehalobacter, Desulfitobacterium, and Dehalococcoides. A large difference in amplification efficiency between the standard curves was observed ...
Many genomic assays rely on a distance-dependent interaction between luminescent labels, such as luminescence quenching or resonance energy transfer. We studied the interaction between electrochemically excited Ru(bpy) 3 2+ and Cy5 in a hybridization assay ...
Sphingomonas paucimobilis B90A is able to degrade the alpha-, beta-, gamma-, and delta-isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynam ...
This paper presents a new electronic methodology to detect DNA hybridization for rapid identification of diseases, as well as food and environmental monitoring on a genetic base. The proposed solution exploits a new (electrical) capacitive measurement circ ...